H1 Biology and H2 Biology
What are the effects of enzyme concentration on the rate of enzyme-catalysed reactions?
Notes from the video ‘H2 Biology Tuition | Effect of Enzyme Concentration on Enzyme-Catalysed Reaction’:
In this Biology tutorial, we would learn about the effects of enzyme concentration on the rate of enzyme-catalysed reactions.
Low Enzyme Concentration
At low enzyme concentration, as enzyme concentration increases, the frequency of effective collisions between the enzyme and substrate increases. This increases the rate of formation of enzyme-substrate complexes which increases the rate of reaction proportionally.
Increasing Enzyme Concentration
As the enzyme concentration increases, enzyme concentration is no longer the only limiting factor, rate of reaction increases at a decreasing rate.
High Enzyme Concentration
Above a certain enzyme concentration, enzyme concentration is no longer limiting and other factors are limiting. Rate of reaction reaches maximum rate. Increasing the concentration of enzymes will not increase the rate of reaction.